Extended Data Fig. 8: RNA ISH and serum markers of toxicity on day 11.
From: Potentiating adoptive cell therapy using synthetic IL-9 receptors
a,b, Representative images of brain and meningeal sections stained with fluorescent probes specific for mouse CAR (red, Cy3), mouse mesothelin (green, FITC) and counterstained with DAPI (blue). CAR-positive cells (white arrows) and mesothelin-positive meningeal cells (purple arrows) indicated in the pia-arachnoid layer of the meninges. Scale bars, 50 μm. c,d, Semi-quantification of CAR T cells and mesothelin-positive cells in stained brain sections. Mean ±SEM, two sections/mouse, three mice/group. *P < 0.05 (two-sided t test with Welch’s correction). e, Serum levels of calcium, phosphorus, potassium, uric acid, and f, cytokine release syndrome (CRS)-associated cytokines on day 11 of treatment. Mean ± SEM, n = 3 mice/group. g, Representative photomicrographs from 3 mice (160 days post-treatment) cured of PDA7940b tumours showing histologically normal leptomeninges, without immune/inflammatory cell infiltrates, covering the temporoparietal region of the cerebral hemispheres. H&E, scale bar: 100 μm.
