Extended Data Fig. 9: Viral RNA dependent RNA polymerase (RdRp) and Spike in human macrophages of human autopsy lungs with SARS-CoV-2 pneumonia (matched to figure 2).
From: Inflammasome activation in infected macrophages drives COVID-19 pathology
Representative fluorescent microscopy images and quantification of colocalization of Spike (S), RNA dependent RNA polymerase (RdRp), human CD68 and DAPI staining in fixed human autopsy lungs with SARS-CoV-2 pneumonia or non-SARS-CoV-2 pneumonia. Quantification was performed based on representative high-power images (40x) in areas showing diffuse alveolar damage. Top panels: Representative of RdRp staining with human CD68; middle panels: Representative of Spike staining with CD68; bottom panels: RdRp and Spike staining in SARS-CoV-2- infected autopsy lungs. Yellow rectangle provides a higher magnification view of the selected area. Pseudo-colours are assigned for visualization. SARS-CoV-2 pneumonia n = 4, non-SARS-CoV-2 pneumonia n = 3 biologically independent specimens.
