Fig. 3: Identification of a potato tuber identity gene.
From: Genome evolution and diversity of wild and cultivated potatoes
a, Venn diagram describing the identification of 229 candidate genes that are involved in regulating stolon or tuber development. b, Conserved CNSs around the Identity of Tuber1 (IT1) locus. tepCNS, conservative score for each site calculated from tomato, Etuberosum and potato genomes; pCNS, conservative score for each site calculated from 45 potato genomes. Grey blocks show potato-specific CNSs. c, Expression pattern of IT1 and its orthologues in five tissues of Etuberosum, tomato and potato species. The 5-kb sequences up- and downstream of IT1 from tomato, Etuberosum and potato were used to infer the phylogenetic relationships. d, Phenotypes of the it1 knockout mutant. Red arrowheads indicate several abnormally developed stolons in the it1 mutant. Scale bars, 10 cm. WT, wild type. e, Comparison of potato tuber development between wild type and the it1 mutant. Scale bar, 5 cm. f, IT1 directly interacts with SP6A, as validated in a yeast-two-hybrid assay. Three independent biological experiments were performed. g, Domain architecture of SP6A in potato and Etuberosum species. AD, Gal 4 activation domain; BD, Gal4 DNA-binding domain; -LW, synthetic dropout medium without Leu and Trp; -LWH, synthetic dropout medium without Leu, Trp and His.
