Extended Data Fig. 2: Mutant fitness quantification.
From: Synonymous mutations in representative yeast genes are mostly strongly non-neutral
a, Fractions of synonymous (yellow) and nonsynonymous (blue) mutants among designed but unobserved mutants and those among observed mutants. Nonsense mutants are not considered. Numbers in the bars are numbers of mutants. The distributions of synonymous and nonsynonymous mutants among the unobserved and observed mutant groups are not significantly different (P > 0.05, Fisher’s exact test). b–f, Correlation between every two of the four replicates in estimated mutant fitness under YPD at 30 °C. The correlation between replicate 1 and replicate 2 is presented in Fig. 1c. Each dot is a mutant and the dotted line indicates the diagonal. Pearson’s correlation r and its associated P-value are presented. Among-genotype sum of squares explains 93.8% of the total sum of squares (one-factor ANOVA)
