Extended Data Fig. 3: Cryo-EM data processing pipeline for dCMGE assembled on nucleosome-capped origin DNA.
From: Mechanism of replication origin melting nucleated by CMG helicase assembly
a. Schematic shows the classification and refinement steps taken to achieve the symmetry-expanded refined CMGE cryo-EM structure. The software used during each processing step is listed. b. Schematic of the classification and refinement pipeline for the C1-refined CMGE dimer. c. Symmetry-expanded refined CMGE structure (coloured) docked into C1-refined CMGE dimer map (grey). Reported resolutions in all schematics are calculated based on the FSC = 0.143 criterion.
