Extended Data Fig. 6: Immune cell subsets in WT and KI mice; related to Figure 3.

a, Schematic of bone marrow cell transplantation experiments. b, Representative dot plots of the frequency of CD45.2 (recipient) and CD45.1 (donor) cells in the peripheral blood of chimeric mice 8 weeks after irradiation and reconstitution. c, Average of motor neuron area calculated in the lumbar region of spinal cord sections. n = 5 mice/group, mean +/− s.e.m. P value calculated using one-way ANOVA Kruskal-Wallis test and Dunn’s post-hoc test (*P = 0.0002). d, Axon areas (left) and number of large calibre axons (right) in the sciatic nerve. n = 6 (WT–WT, KI–KI, KI–WT) and 7 (WT–KI) mice per group; mean +/− s.e.m. P value calculated using one-way ANOVA Kruskal-Wallis test and Dunn’s post-hoc test (*P = 0.026, ns=not statistically significant). Chimeric mice in (c,d) were analysed at 12-13 months after reconstitution. e, Peripheral blood cells from two mice of the indicated group were pooled and analysed by mass cytometry. Left, Details of markers and cell subsets identified by SPADE (spanning-tree progression analysis for density-normalized events) analysis (WT–WT group is shown). Right, SPADE analysis showing the distribution of immune cells into 4 main populations. Size and colour of circles are dependent on the number of cells and the median intensity of expression for each marker, respectively. Red circles highlight PD-1⁺ CD8 T cells.