Extended Data Fig. 8: Absence of T cell signature in Fus^P517L/WT and SOD1^G93A mice.

a,d, Cell suspensions from brain, spinal cord, spleen, and peripheral blood of 22–24 month-old Fus^P517L/WT and Fus^WT/WT littermates were analysed by spectral flow cytometry. a–c, t-SNE of total live CD45⁺ cells (a) and frequency of TCRβ⁺ CD8⁺ and CD4⁺ T cells expressing CD62L, CD44, PD-1⁺ and Foxp3 (b,c) from the spinal cord of Fus^P517L/WT and Fus^WT/WT mice after concatenation of all of the 5 samples/group. d, Frequency of CD8 T cell subsets in the indicated organs from the 2 genotypes (2 independent experiments, 5 individual mice/group; mean +/− s.e.m.). e, Frequency of CD8 T cell subsets in the indicated organs from 3-4 month-old non-carrier and SOD1^G93A mice by spectral flow cytometry analysis (2 independent experiments, n = 5 (spleen), n = 6 (brain, WT blood) and n = 7 individual mice/group; mean +/− s.e.m.). N= naïve, CD62L⁺CD44⁻; CM= central memory, CD62L⁺CD44⁺; EM= effector memory CD62L⁻CD44⁺. T cells are gated on live CD45^high CD19⁻NK1.1⁻CD11b⁻CD11c⁻TCRγδ⁻TCRβ⁺ CD8⁺ or CD4⁺ cells.

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