Extended Data Fig. 1: Characterization of KI mice.
From: Clonally expanded CD8 T cells characterize amyotrophic lateral sclerosis-4
a, Average latency to fall (sec) from an accelerating rotarod of WT and KI mice tested at 6 and 8 months of age (2 independent experiments, n = 11 (WT 6 months and KI 8 months) and n = 13 (WT 8 months and KI 6 months) individual mice per group; mean +/− s.e.m.). P value calculated using the one-way Repeated Measures ANOVA (**P = 0.0012) and Bonferroni’s post-test. (b,c) Bone marrow derived dendritic cells (BMDCs) from Setx WT and KI (b,c), and HET and knockout (KO) (b) mice were infected with Citrobacter rodentium (multiplicity of infection = 10, b) or stimulated with 5 μg/ml of cGAMP (c). Bar graphs show the relative induction of the indicated transcripts by RT–qPCR analysis at 2 h post-infection or cGAMP stimulation in KO and KI cells normalized to their controls (2 independent experiments, n = 4 mice/group, mean +/− s.e.m.). No expression of the indicated genes was detected in uninfected cells. d, Concentration of the indicated cytokines measured by Luminex Assay in the serum of KI and WT (8-9 months old) mice (1 out of 2 independent experiments, n = 6 (WT) and n = 5 (KI) mice/group, mean +/− s.e.m.). e, RT–qPCR analysis for the indicated inflammatory genes in the spinal cord of 10 months old WT and KI mice (Log2 relative expression against Actb, 2 independent experiments, n = 5 mice/group, mean +/− s.e.m.). f, Absolute numbers of indicated immune cell subsets in the spleen of 8-9 month-old KI and WT mice. pDC: plasmacytoid dendritic cells; cDC: conventional dendritic cells. g, Expression of the activation marker CD86 in cDC and CD11b+ cells from the spleen of 8-9 months old WT and KI mice. (f,g) 2 independent experiments, n = 4 mice/group, mean +/− s.e.m.
