Extended Data Fig. 3: Immune cell subsets in WT and KI mice; related to Figure 1.
From: Clonally expanded CD8 T cells characterize amyotrophic lateral sclerosis-4
a,b, Peripheral blood cells from 8-month-old WT and KI mice were analysed by mass cytometry. a, t-SNE plots after gating on live CD45high (top) and CD19−NK1.1−CD11b−CD11c−TCRβ+ (bottom). b, Frequency of PD-1+ expressing cells after gating on CD44+ CD8+ T cells. Three mice/group were pooled for mass cytometry analysis. c, Gating strategy for flow cytometry analysis of CD8 T cells in the CNS. d, Frequency of PD-1+ CD8 T cells in the spleen and lungs of WT and KI mice at the indicated ages (2 independent experiments, 4 mice/group; mean +/− s.e.m.). e, Frequency of PD-1+ (left) and Foxp3+ (right) CD4 T cells in the peripheral blood of WT and KI mice at the indicated ages (3 independent experiments, n = 6 (left) and 2 independent experiments, n = 4 (8 months) and n = 6 (right) individual mice/group. SB=super bright.
