Extended Data Fig. 4: PD-1+ CD8 T cells are effector memory T cells activated in an antigen manner.
From: Clonally expanded CD8 T cells characterize amyotrophic lateral sclerosis-4
a, Expression of indicated surface markers in CD8 T cells from the spinal cord (top), brain (middle) and peripheral blood (bottom) of 10–12-month-old KI mice after gating on PD-1+ and PD-1− CD8 T cells. Histograms are representative of 3 independent experiment (n = 6 mice). b, Median fluorescence intensity (MFI) of the indicated markers in CD44+CD62L− PD-1+ and PD-1− CD8 T cells isolated from the spinal cord (top) and blood (bottom) of 10–12-month-old KI mice (2 independent experiments, n = 6 (blood TIGIT and TOX), n = 5 (spinal cord TIGIT and TOX), n = 4 (TCF-1) individual mice/group, mean +/− s.e.m.). P value calculated using a two-tailed unpaired t-test with Welch’s correction (spinal cord: ***P = 0.0007; *P = 0.045; **P = 0.0066; blood: ***P = 0.0004 (TOX); ***P = 0.0002 (TIGIT and TCF-1). c, KI mice were crossed to OT-I mice and the offspring analysed at 10 months of age. Dot plots from spinal cords, SC (left), and bar graphs (right) show the frequency of PD-1+ among OVA specific (gated on Vα2+Vβ5+) and non-TCR transgenic (gated on Vα2−) CD8 T cells (3 independent experiments, n = 6, brain and spinal cord, and n = 5, blood, individual mice; mean+/− s.e.m.). P value calculated using a two-tailed unpaired t test with Welch’s correction. **P = 0.0031 (brain); ***P = 0.0004 (SC); ***P = 0.0003 (blood). eF=eFluor.
