Extended Data Fig. 7: cBAF cooperates with MYC to restrict CD8⁺ T_mem differentiation.

a, b, Naïve CD8⁺ T cells from Rosa26^creERT2Myc^fl/fl and Myc^fl/wt littermates were treated with 4OHT overnight in IL-2-containing medium prior to activation with anti-CD3/CD28 plus ICAM1 for 36 h. a, Volcano plot of gene profiles in Myc^−/− versus WT activated CD8⁺ T cells as determined by RNA-seq analysis. BAF components are highlighted (n = 5 biological replicates). b, Immunoblot of BAF components in WT and Myc^−/− CD8⁺ T cells. c, d, Naïve CD8⁺ T cells from WT and Myc^+/− mice were activated by anti-CD3/CD28 plus ICAM1 for 28 h before immunofluorescent staining. Representative image (c) and quantification (d) of conjoined WT and Myc^+/− daughter CD8⁺ T cells that were fixed and stained for Hoechst (blue), ATAC-see (red), Lamin B1 (green) and Tubulin (white). Scale bar: 5 μm. Asymmetric index = (fluorescence intensity in daughter cell 1−fluorescence intensity in daughter cell 2)/(fluorescent intensity in daughter cell 1+ fluorescence intensity in daughter cell 2). e, Quantification of the normalized CX3CR1^highKLRG1^high (left) or CD27^highCXCR3^high (right) ratio in OT-I cells at day 7.5 post Lm-Ova infection. sgNTC (GFP⁺ spike)- and the indicated constructs (GFP⁺Ametrine⁺)-transduced OT-I cells were mixed at a 1:1 ratio and transferred to C57BL/6 hosts followed by Lm-Ova infection. The normalized cell ratio was calculated by dividing the proportions of CX3CR1^highKLRG1^high cells or CD27^highCXCR3^high cells in GFP⁺Ametrine⁺ cells by their proportions in GFP⁺ spike cells. Data are representative of one (a) or two (b, c) or compiled from two independent experiments (d, e). Data are shown as mean±s.e.m. *P < 0.05, **P < 0.01, ***P < 0.001; NS, not significant; two-tailed unpaired Student’s t-test (d, e), two-sided Wald test and adjusted with Benjamini-Hochberg procedure (a).

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