Extended Data Fig. 2: Deficiency in SWI/SNF promotes bona fide Tmem formation.
From: cBAF complex components and MYC cooperate early in CD8+ T cell fate
a, Diagram of experimental approach to evaluate Tmem function. p.i., post-infection. b, Quantification of numbers of OT-I cells expressing the indicated sgRNA in multiple tissues at day >30 post-infection (n = 5 mice per group). c, Flow cytometry analysis (left) and quantification (right) of the mean fluorescence intensity (MFI) of CellTrace Violet (CTV) dilution in donor-derived OT-I cells. Splenic Tmem OT-I cells transduced with either sgNTC (GFP+ spike cells) or the indicated sgRNA (Ametrine+) were isolated from mice that were challenged with Lm-Ova at day >30 prior, labelled with CTV, mixed at a 1:1 ratio, and transferred to Rag1-deficient recipients. Cells were analysed at day 7 post-transfer (n = 3 mice per group). d, e, Splenic Tmem OT-I cells transduced with sgNTC (GFP+ spike cells) or the indicated sgRNA (Ametrine+) were isolated from mice that were challenged with Lm-Ova at day >30 prior, mixed at a 1:1 ratio, and transferred to naïve C57BL/6 recipients followed by Lm-Ova re-challenge. The cells were analysed at day 6 after re-challenge. d, Flow cytometry plot and quantification of cells expressing both interferon-γ (IFNγ) and TNFα after ovalbumin peptide stimulation for 5 h in the presence of monensin (n = 5 per group). e, Quantification of Granzyme B (GZMB) MFI (n = 5 per group). Data are compiled from at least two independent experiments (b–e). Data are shown as mean±s.e.m. *P < 0.05, **P < 0.01; two-tailed paired Student’s t-test (b, c, d, e).
