Extended Data Fig. 5: The cBAF complex shares binding sites with MYC in T cell differentiation-associated chromatin loci.
From: cBAF complex components and MYC cooperate early in CD8+ T cell fate
a–d, WT and Arid1a−/− naïve CD8+ T cells were activated by anti-CD3/CD28 plus ICAM1 for 36 h, and first-division CD8+ T cells were sorted for CUT&RUN assay. a, Venn diagram of the overlap between CUT&RUN peaks in WT first-division CD8+ T cells for ARID1A and BRG1 (n = 2 biological replicates). b, Relative locations of MYC peaks to BAF components peaks in chromatin. c, Enrichment analysis of BRG1 and MYC shared binding sites in first-division WT CD8+ T cells using Hallmark gene sets (upper) and Gene ontology gene sets (lower). The co-bound sites were annotated to the nearest genes and enrichment was calculated with over-representation approach. d, Genome browser tracks of MYC and cBAF shared binding sites at Gzmb, Il2ra and Tbx21 loci. The overlapping peaks are highlighted. Data are representative of one experiment (a−d). The P-value was calculated by hypergeometric distribution, one-sided and adjusted with Benjamini-Hochberg procedure (c).
