Extended Data Fig. 3: Flow chart of cryo-EM data processing for NTCP-Fab complex. | Nature

Extended Data Fig. 3: Flow chart of cryo-EM data processing for NTCP-Fab complex.

From: Structural insights into the HBV receptor and bile acid transporter NTCP

Extended Data Fig. 3

a, Schematic flow chart of the classification and refinement procedures to resolve the NTCP-Fab complex structure. b, Representative raw micrograph of NTCP-Fab complex. c, Gallery of two-dimensional class averages, with a window size of 232 Å. d, Final three-dimensional density map coloured by local resolution of the NTCP-Fab complex. e, Euler angle distribution of all particles included in the calculation of the final three-dimensional reconstruction. f, Fourier shell correlation curve of the globally refined NTCP-Fab complex after the post-processing with RELION. The red line intercepts the y axis at a FSC value of 0.143.

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