Extended Data Fig. 5: Sequence alignment of the NTCP through the species.

Sequence alignment of NTCP of Human, Chimpanzee, Monkey, Treeshrew, Rat and bacterial ASBT, calculated with CLUSTALW. The bars mark the locations of helices in NTCP, and are coloured as in Fig. 1c. Putative residues interacting with Na-1 and Na-2 are highlighted with orange and pink, respectively. Residues forming the TCA / preS1 binding pocket are highlighted in purple. G158 and S267 are highlighted in blue; these residues affect the species specificity of HBV infection among great apes and Old-World monkeys. Other residues known to control species specificity are highlighted in green. Key residues important for TCA and preS1 binding, and NTCP function, are highlighted in yellow.