Fig. 2: Robust viral replication of WA1 and Delta, but not Omicron, in airway cells from mice and humans.
From: Limited cross-variant immunity from SARS-CoV-2 Omicron without vaccination
a, Plaque assay titres from the upper airway (nasal turbinates and bronchus) of WA1-infected, Delta-infected and Omicron-infected mice at the indicated time points. Data are shown as the average ± s.e.m. analysed by the two-tailed unpaired Student’s t-test. Each dot represents an infectious virus titre in an individual mouse at 2 d.p.i. (n = 5), 4 d.p.i. (n = 5) and 7 d.p.i. (WA1 infection group n = 2, Delta n = 2 and Omicron n = 5). b, Plaque assay titres from the lungs of infected mice at the indicated time points. Data are shown as the average ± s.e.m. at each time point and were analysed by the two-tailed unpaired Student’s t-test. Each dot represents infectious virus titre in individual mice at 2 d.p.i. (n = 5), 4 d.p.i. (n = 5) and 7 d.p.i. (WA1 infection group n = 2, Delta n = 2 and Omicron n = 5). c, Plaque assay titres from supernatants of infected human airway organoids (multiplicity of infection (MOI) of 1). Data are shown as the average ± s.e.m. Each dot represents an independent experiment using human lung airway organoids generated at 24 h (n = 2) and 48 h (n = 3). h.p.i., hours post-infection. d, Plaque assay titres from supernatants of infected A549-ACE2 cells (MOI of 0.1). n = 2 represents infectious virus titres in independent experiments.
