Extended Data Fig. 6: Mutation of three NFIL3 binding sites in the –165 kb Zeb2 enhancer abrogates cDC2 development (Part II).
From: Ablation of cDC2 development by triple mutations within the Zeb2 enhancer
a, Representative flow plots showing migratory and resident cDCs in mesenteric lymph nodes (MLNs) from WT and Δ1+2+3 naïve mice or mice infected with H. polygyrus (H.p.) for 14 days. b, Frequency of migratory CD24– CD172a+ cDC2 and resident CD172a+ cDC2 in MLNs from WT and Δ1+2+3 naïve mice or mice infected with H.p. for 14 days. Data are pooled from seven independent experiments (n = 9 for naïve mice and n = 5 for H.p. infected mice). c, Frequency of migratory CD103– CD11b+ cDC2 and resident CD172a+ cDC2 in SLNs from WT and Δ1+2+3 mice. Data are pooled from three independent experiments (n = 3 for each genotype). d, Frequency of CD103– CD11b+ cDC2 in small intestine lamina propria from WT and Δ1+2+3 mice. Data are pooled from four independent experiments (n = 4 for each genotype). e, Representative flow plots showing small intestine lamina propria cDCs in WT and Δ1+2+3 mice. f, Frequency and number of splenic pDC in WT, Δ1, Δ1+2, Δ1+3 and Δ1+2+3 mice. Data are pooled from five independent experiments (n = 12 for WT, n = 11 for Δ1, Δ1+2, Δ1+3, and n = 8 for Δ1+2+3 mice). g, Frequency of BM pDC in WT and Δ1+2+3 mice. Data are pooled from three independent experiments (n = 7 for each genotype). h, IFN-α, measured from splenic pDCs stimulated for 16 h with CpG-A 2216, or left unstimulated. Data are pooled from two independent experiments (n = 4 for each genotype). Mean ± s.d.; b, c, h: unpaired, multiple t tests with Welch correction; d, g: unpaired, two-tailed Mann−Whitney test; f: Brown−Forsythe and Welch ANOVA with Dunnett’s T3 multiple comparisons test.
