Extended Data Fig. 7: Mutation of three NFIL3 binding sites in the –165 kb Zeb2 enhancer abrogates monocyte development.
From: Ablation of cDC2 development by triple mutations within the Zeb2 enhancer
a, Representative flow plots showing monocytes among CD45+ peripheral blood cells from WT, Δ1, Δ1+2, Δ1+3 and Δ1+2+3 mice. b, Representative flow plots showing cMoPs and monocytes (Mo) in BM of WT and Δ1+2+3 mice. Data shown are one of five similar experiments. c, Frequency of peripheral blood monocytes, splenic monocytes, and number of splenic monocytes in WT, Δ1, Δ1+2, Δ1+3 and Δ1+2+3 mice. Data are pooled from two independent experiments for peripheral blood monocytes and five independent experiments for splenic monocytes (peripheral blood monocytes: n = 13 for WT, n = 10 for Δ1, n = 8 for Δ1+2, n = 9 for Δ1+3 and n = 7 for Δ1+2+3 mice; splenic monocytes: n = 11 for WT, n = 7 for Δ1, n = 4 for Δ1+2, n = 7 for Δ1+3 and n = 8 for Δ1+2+3 mice). d, Representative flow plots showing splenic B cells and T cells in WT and Δ1+2+3 mice. e, Frequency of splenic B cells and T cells in WT, Δ1, Δ1+2, Δ1+3 and Δ1+2+3 mice. Data are pooled from three independent experiments (n = 9 for WT, n = 7 for Δ1, Δ1+2, Δ1+3, and n = 5 for Δ1+2+3 mice). WT versus Δ1+2+3 B cell P = 0.9238, WT versus Δ1+2+3 T cell P = 0.6980. f, Representative flow plots showing monocytes and macrophages in small intestine lamina propria from WT and Δ1+2+3 mice. g, Frequency of small intestine lamina propria monocytes and macrophages in WT and Δ1+2+3 mice. Data are pooled from four independent experiments (n = 4 for each genotype). Mean ± s.d.; NS, not significant; c, e: Brown−Forsythe and Welch ANOVA with Dunnett’s T3 multiple comparisons test; g: unpaired, two-tailed Mann−Whitney test.
