Extended Data Fig. 9: Myeloid and lymphoid pathways of pDC development are distinguished by different requirements in the –165 kb Zeb2 enhancer.

a, Representative flow plots showing pDCs and cDCs differentiated from sort purified CDPs and MDPs of WT and Δ1+2+3 mice, assessed after 5 days of culture with Flt3L. Data shown are one of three similar experiments. b, Representative flow plots showing in vivo developmental potential of CDPs and MDPs from WT and Δ1+2+3 mice. Recipient spleens were analyzed for the presence of CD45.2⁺ donor-derived pDCs and cDCs. Data shown are one of two (CDP) or three (MDP) similar experiments. c, Representative flow plots showing pDCs differentiated from sort purified CMPs and CLPs of WT and Δ1+2+3 mice, assessed after 7 (CMPs) or 5 (CLPs) days of culture with Flt3L. Data shown are one of two (CMP) or three (CLP) similar experiments. d, Representative flow plots showing CDPs (CD115⁺ CD127^–), IL-7R⁺ LPs (CD115^– CD127⁺) and IL-7R^– CSF1R^– NPs (CD115^– CD127^–) in the BM of WT and Δ1+2+3 mice (pre-gate: lineage^– CD16/CD32^– CD135⁺ CD117^int-neg cells). Data shown are one of three similar experiments. e, Representative flow plots showing pDCs differentiated from sort purified CDPs, IL-7R⁺ LPs and IL-7R^–CSF1R^– NPs (as in d) of WT and Δ1+2+3 mice, assessed after 4 days of culture with Flt3L. Data shown are one of three similar experiments.