Extended Data Fig. 5: Comparison of RNA-seq expression profiles in lung tissues from Adar1^−/mZα Mavs^WT/− and control mice.

Comparison of lung RNAseq data from Adar1^−/mZα Mavs^WT/− (n = 5), Adar1^WT/mZα (n = 5) and wild type C57BL/6N mice (n = 5) at 15 weeks of age. Heatmap shows 678 genes differentially expressed between Adar1^−/mZα Mavs^WT/− mice and the two control groups combined (p ≤ 0.05, q ≤ 0.05, ≥ 2-fold-change), with 399 of these upregulated in the former (Supplementary Data Table 3). Columns represent individual mice, hierarchically clustered according to differential gene expression. Tables show GO functional annotation of the genes found upregulated and downregulated in this comparison, performed with g:Profiler (https://biit.cs.ut.ee/gprofiler). P values for differential expression analyses were calculated with Qlucore Omics Explorer using two-sided t-tests and with the q value for false discovery rates (FDR) set to 0.05. Calculation of q values was adjusted for multiple hypothesis testing using the Benjamini-Hochberg method. P values for pathway analyses were calculated with g:Profiler using hypergeometric distribution tests and adjusted for multiple hypothesis testing using the g:SCS (set counts and sizes) algorithm, integral to the g:Profiler server (https://biit.cs.ut.ee/gprofiler).