Extended Data Fig. 2: The paraventricular hypothalamus controls leukocyte homing to the bone marrow during stress.
From: Brain motor and fear circuits regulate leukocytes during acute stress
a, Quantification of plasma corticosterone levels in adrenalectomized (ADX) and sham-operated mice at baseline and after 4 h of restraint stress (n = 6 mice per group). Two-way ANOVA. b, Quantification of plasma corticosterone levels 1 h and 4 h after intraperitoneal injection of vehicle or corticosterone (5mg/kg) into WT mice (n = 5 mice per group). Two-way ANOVA. c, Transcript expression of Nr3c1 (GR) on sorted blood leukocytes expressed as ΔCT compared to the house-keeping gene Gapdh (CTmean Nr3c1 - CTmean Gapdh) (sorted cells from n = 4 samples, each sample pooled from n = 4 WT mice). One-way ANOVA. d, Quantification of circulating blood leukocytes in mice with CX3CR1Cre-driven knockout of the glucocorticoid receptor (CX3CR1Cre+/−:GRflox+/+) and control mice (GRflox+/+) at baseline and after 4 h of restraint stress (n = 3 CX3CR1Cre+/−:GRflox+/+ mice, and n = 4 GRflox+/+ mice). Two-way ANOVA. e, Gating strategies and percentages of cells expressing enhanced yellow fluorescent protein (EYFP+) within the indicated blood leukocyte populations in CD19Cre+/−:EYFP+/- reporter mice (n = 4), LysMCre+/−:EYFP+/− reporter mice (n = 12), CD4Cre+/−:EYFP+/− reporter mice (n = 3), and CX3CR1Cre+/−:EYFP+/− reporter mice (n = 2). Data are mean ±s.e.m.; ★P < 0.05, ★★P < 0.01, ★★★P < 0.001, statistical tests used as indicated.
