Fig. 1: Cryo-EM structure of the ligase complex.
From: A peroxisomal ubiquitin ligase complex forms a retrotranslocation channel
a, Gel-filtration profile of the Fab-bound T. thermophilus (T.t.) ligase complex (Pex2 (140–495), Pex10 (1–454) and Pex12 (1–439) with an N-terminal streptavidin-binding peptide (SBP) tag) in nanodiscs or in digitonin. b, The peak fractions between the dashed lines in a were analysed by SDS–PAGE and Coomassie blue staining, as well as by negative-stain EM. MSP1D1 is the scaffold protein of the nanodiscs. The white arrow indicates the bound Fab. The results are representative of three biological repeats. For gel source data, see Supplementary Fig. 1. MW, molecular weight. c, Cryo-EM density map of the ligase complex with bound Fab, with views from the side and the cytosol. The map was sharpened with a B factor of −80.0 Å2 and is shown contoured at a level of 0.041. CytD, cytosolic domain; Fv, variable domain of the Fab; TMD, membrane-embedded domain. d, Model of the ligase complex. The boundary of the detergent micelle is indicated.
