Extended Data Fig. 6: Mutation frequency and percentage of hypermutated E/SEs in cases of primary DLBCL.
From: Super-enhancer hypermutation alters oncogene expression in B cell lymphoma
a. Venn diagrams showing the overlap between E/SEs identified in normal GC B cells, GCB-DLBCL cell lines and ABC-DLBCL cell lines. The shadowed area marks the subset of E/SEs interrogated in each of the analyses shown in panels b and c (same row), and the corresponding number is given for each subset inside the diagram. The total number of E/SEs identified in GC B cells, GCB-DLBCL cell lines and ABC-DLBCL cell lines appears outside the Venn diagram, in brackets. b, c. Sample-based mutation frequency (b) and percentage (c) of hypermutated E/SEs in primary DLBCL specimens grouped based on phenotypic subtypes (UNC, unclassified; ND, not determined). The analysis of different regions (corresponding to those highlighted in the aligned Venn diagram) is displayed in different rows, and data for “shared” E/SEs (Fig. 1d, e) are shown for comparison on the top row, as these regions emerged as harbouring the highest mutation frequency and % of hypermutated cases in DLBCL. In the graphs, each dot denotes one primary DLBCL sample, and mutation frequencies are expressed as fold changes vs. background, calculated in the same sample as described in Methods. The grey dashed line in panel b represents the background mutation frequency, set at 1 for each sample (see Methods). All p-values were calculated by two-sided Wilcoxon rank-sum test after BH correction.
