Extended Data Fig. 3: Histone modification pattern of representative intragenic and TSS-distal SEs targeted by mutations in DLBCL.

a. ChIP–seq tracks of H3K4me3, H3K4me1, H3K27Ac, and H3K27me3 at representative intragenic SHM-targeted SEs in normal GC B cells vs. primary B cell lymphomas. Enrichment is visualized as reads per bins per million bps (BPM), and the genomic coordinates of the region shown (hg19) are provided at the bottom, with the annotated coding gene/s (RefSeq accession No: NM_001706 for BCL6, NM_000633 for BCL2, NM_003467 for CXCR4, and NM_016734 for PAX5). Green horizontal bars below the H3K27Ac tracks indicate regions identified as SEs by ROSE. The dotted square indicates the hypermutated region. In the bottom panel, distribution and number of mutations identified in cases of primary DLBCL. b. ChIP–seq tracks of H3K4me3, H3K4me1, H3K27Ac, and H3K27me3 at representative TSS-distal, SHM-targeted SEs in normal GC B cells. H3K27Ac is also shown for the DLBCL cell line LY1. Enrichment is visualized as reads per bins per million bps (BPM), and green horizontal bars below the H3K27Ac tracks indicate regions identified as SEs by ROSE. The cartoon on top provides a broader view of the genomic region expanded below (dotted lines), with annotated coding genes represented as solid boxes, intergenic regions represented as lines, and their promoter orientation indicated as an arrow (not in scale). The exact genomic coordinates (hg19) of the region magnified are provided at the bottom, and the distribution of somatic mutations found across the region in DLBCL (cell lines and primary cases) is plotted below the gene/s track.