Extended Data Fig. 3: Gene expression analysis after ecdysteroid injections.
From: A male steroid controls female sexual behaviour in the malaria mosquito
3D20E injections (0.63 µg) induce significantly stronger expression of MISO compared to the same amount of 20E. 3D20E injections also specifically or more potently activate the expression of EcR isoform A (EcR-A), and the mating-induced genes AGAP006421, AGAP004263 and HPX15, while they repress the EcR isoform EcR-B. 20E injections specifically activate the yolk protein gene Vg and HR4, and more potently induce HR3 expression (Data are presented as means ± s.e.m. that were derived from three biological replicates of pools of 10 mosquitoes each (HR4 data were from four biological relications of pools of 10 mosquitoes each), unpaired, two-sided, FDR-corrected t-tests were conducted for 20E vs 3D20E comparisons; statistical significance above each bar denotes comparison with 10% EtOH injection: unpaired t-test, two-sided). See Supplementary Table 3 for P values
