Extended Data Fig. 4: Male survival is affected by kinase silencing.
From: A male steroid controls female sexual behaviour in the malaria mosquito
(a) Survival of males is negatively affected by silencing of EcK1 and EcK2 but not EPP. Survival rate is significantly lowered in EcK1-silenced males (left) and Eck2-silenced males (middle), but not in EPP-silenced (right) males relative to dsGFP controls (means ± s.e.m., n indicates the number of biologically independent mosquito samples, Mantel-Cox test, two-sided). (b–c) Recombinant EcK2 (rEcK2) efficiently phosphorylates (b) 20E but not (c) 3D20E, as determined by HPLC-MS/MS. Means ± s.e.m. were derived from three in vitro assay replicates. Negative controls (DHFR and heat-inactivated, H.I., rEcK2) do not phosphorylate 20E or 3D20E. Schematic representations of the reactions catalyzed by EcK2 are provided
