Extended Data Fig. 1: Protein purification and dsRNA-processing activity of Dcr-2–Loqs-PD.
From: Structural insights into dsRNA processing by Drosophila Dicer-2–Loqs-PD
a–b, Size exclusion chromatography results of Dcr-2–Loqs-PD and Dcr-2–Loqs-PD/50-bp dsRNA complex. c, SDS-PAGE of the protein fractions from b. d, Cleavage assays of Dcr-2–Loqs-PDFL (1.2 μM) with 50-bp dsRNA (1.2 μM), in the cleavage assay buffer (25 °C). Products were resolved on a 12% polyacrylamide denaturing gel. For gel source data, see Supplementary Figure 1.
