Extended Data Fig. 6: Immunohistological analysis of STMN1 enrichment in human imGCs across the lifespan.
From: Molecular landscapes of human hippocampal immature neurons across lifespan
a-d, Sample confocal images (a, b) and quantifications (c, d) of imGCs in the human dentate gyrus across the lifespan. Asterisks indicate DCX+ or CALB1- among STMN1+PROX1+ GCs (a, b). Box plots similar as in Fig. 1g (n = 4 subjects each group) (c, d). The immunohistological signal of STMN1 was noticeably more robust than that of DCX in adult specimens. e, f, Sample confocal images showing NEUROD1+, NEUN+ (e), S100B−, or OLIG2− (f) among STMN1+PROX1+ imGCs in infant or adult human dentate gyrus, confirming their neuronal identity. Asterisks indicate STMN1+PROX1+ imGCs (n = 1 specimen for each immunostaining). All scale bars, 10 µm.
