Extended Data Fig. 2: YAP/TAZ in dermal fibroblasts control dermal and ECM homeostasis.
From: YAP/TAZ activity in stromal cells prevents ageing by controlling cGAS–STING
a, Representative in situ hybridization for Cre recombinase (CreER) in dorsal skin sections of a Col1a2-CreER mouse, showing specific staining in dermal fibroblasts (Scale bar, 50 µm). b, Quantification of Vimentin-positive cells in the dermal layer of skin sections from mice of the indicated genotypes. Data (n = 4 mice for each column) are presented as mean ± s.d.; P values are derived from one-way ANOVA with Dunnett’s multiple comparison test. Related to Fig. 2b. c, Quantification of Cdkn1a-positive cells in the dermal layer of skin sections from mice of the indicated genotypes. Data (n = 4 per condition) are presented as mean ± s.d.; P values are derived from one-way ANOVA with Dunnett’s multiple comparison test. Related to Fig. 2c. d, e, Representative birefringence images (d, Scale bar, 100 µm) and quantification (e) of picrosirius red staining on dorsal skin sections from mice of the indicated genotypes. In d, the red-to-green gradient reflects the decreasing thickness of collagen fiber bundles. In e, red is the quantification of thicker collagen fibers appearing as red or yellow in birefringence; green is the quantification of thinner fibers appearing as green in the same conditions. Data in e (n = 4 mice for each column) are presented as mean + s.d., 25 areas were analyzed per condition. f, Violin plots from sc-RNA seq data showing the expression distribution of Cdkn1a and Btg2 in freshly dissociated dermal fibroblasts of young control (n = 2916 cells), young Y/T cKO (n = 759 cells) and old control (n = 1413 cells) mice. Red lines denote the median, black lines denote quartiles. P values are derived from Kruskal-Wallis test. g, Immunoblotting analysis of YAP in tail tip biopsies from wild-type (WT Control) and R26-rtTAM2; Col-tetO-YAPS127A mice treated with intermittent doxycycline regime to sustain YAP activity. Mice were analyzed under doxycycline (Doxy Pulse) or during the off-doxy period, two or four days after the last doxy pulse (Wash out d2 and Wash out d4, respectively; see Methods). GAPDH served as loading control.
