Extended Data Fig. 7: Additional behavioural analysis of ablated animals.
From: Visual recognition of social signals by a tectothalamic neural circuit
a, Definition of short-range repulsion. Top left shows a representative neighbour map for one of 45 similar control animals interacting with a dot of 4 mm diameter. Colour map as in Fig. 4e. Top right and bottom left show 24 radial line scans of smoothed neighbour density. Each scan begins at the centre of the map. Black dots label the maximum of each scan. Bottom right shows the mean of all line scans in black. Repulsion is quantified as the area above the mean line, left of the peak (blue shading). This defines the reduction of attraction at the centre of the map. Dotted line in bottom panels indicates baseline neighbour density (random distribution). b, Attraction and neighbour density are strongly reduced in 81c:NTR ablated animals. Short-range repulsion is intact. N = 15 ablated, 45 control animals. Data represent individual animals and mean (attraction) or median (repulsion). Neighbour maps show mean probability of finding the stimulus in space with the focal animal at the centre of the map, heading up. Values represent ratios, relative to time-shuffled data. Each map is 60 x 60 mm. ***: p < 0.001/7, **: p < 0.01/7, ns: p > 0.05/7. Two-sided student’s t-test for attraction, Mann-Whitney U test for repulsion, alpha values Bonferroni corrected for 7 comparisons. See figure source data for individual p-values. c, Loom induced startle responses are intact in 81c:NTR ablated animals. Same animals as in Fig. 4c, d. Data represents mean, shading represents 1SD. N = 13 ablated, N = 15 control animals. d, Shoaling with real conspecifics is disrupted in 81c:NTR ablated animals. Fish as in Fig. 4c, d +1 additional animal per group tested in pairs. N = 14 ablated animals, tested as 7 pairs, N = 16 control animals, tested as 8 pairs. e, Quantification of control behaviours in animals shown in Fig. 4c, d, i, and k. Refer to legend of Fig. 4 and figure source data for additional statistical information. f, Attraction shown separately for 81c:NTR-RFP ablation +/− RFP and MTZ +/− control groups, same data as 2 mm dots in b. RFP: red fluorescent protein indicates expression/ no expression of nitroreductase, respectively. MTZ: Metronidazole incubation/ DMSO control, respectively. N = 15 animals in each group. g, Attraction shown separately for s1026tEt:NTR-RFP ablation +/− RFP and MTZ +/− control groups, same data as in e. N = 7 animals in each group. Data in d-g show individual animals or pairs and mean. Error bars are 1SD. p: Two-sided student’s t-tests, uncorrected. d: Cohen’s d. Scale bar: 20 mm.
