Extended Data Fig. 8: Evoked neuronal activity in SAGFF(lf)81c ablated animals.

a, Juvenile expression of SAGFF(lf)81c:Gal4, UAS:NTR-mCherry and s1026tEt, UAS:NTR-mCherry relative to BPN KDE (yellow outline). Markers were imaged in separate 21 dpf fish and registered to the juvenile standard brain. SAGFF(lf)81c: average of 3 animals. s1026tEt: average of 2 animals. elavl3:H2B-GCaMP6s: mapzebrain reference channel. Merged view is shown in 3 orthogonal axes. White crosshairs indicate the orthogonal planes. b, Larval expression of SAGFF(lf)81c:Gal4, UAS:NTR-mCherry relative to BPN KDE (yellow outline) and cort HCR. Markers were imaged in separate 5–7 dpf fish and registered to the mapzebrain standard brain. 81c: average of 4 animals SAGFF(lf)81c:Gal4, UAS:NTR-mCherry. elavl3: mapzebrain reference channel. cort: average of 3 animals, HCR label. Merged view is shown in 3 orthogonal planes. White crosshairs indicate the orthogonal planes. Also see Video S3. c, Additional analysis of Fig. 4e–h. Number of neurons with mean dF/F above the 90th percentile in DT for bout-like dot motion, continuous dot motion, as well as in pretectum for translational gratings and looming stimuli of 81c:NTR ablated and control animals, respectively. Ablated animals show significantly less responding neurons for all stimuli compared to controls. Individual data points denote animals. Color code of individual neurons is z-scored mean dF/F per stimulus. N = 7 ablated, 9 control animals. d, Same experiment as in Fig. 4e–h, and Extended Data Fig. 8c, but in 7dpf larvae. Here, significant reduction of responding neurons in 81c:NTR ablated animals is only observed for DT-BPNs and bout-like dot motion in DT, whereas continuous dot motion, translational gratings and looming do not evoke significantly less responses in the pretectum of ablated animals. N = 8 ablated, 9 control animals. Error bars: 1SD. Scale bars: a, b, 100 µm; d. p-values in c,d result from two-sided Mann-Whitney U test (uncorrected).

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