Extended Data Fig. 2: Quantification of c-fos and cort HCR labelling.
From: Visual recognition of social signals by a tectothalamic neural circuit
a, Dorsal thalamic plane showing HCR labelling of c-fos and cort in one animal after interaction with a bout-like motion dot stimulus. Expression occurs in the same regional cluster (DT) but only one cell expresses both markers (arrowhead). Representative for 6 examined animals. b, Left: Expression of cort and c-fos induction by shoaling stimuli localize to the same area. elavl3, cort and c-fos were co-labelled in the same animals. A single horizontal imaging plane at the centre of the DT cluster is shown. elavl3 and cort channels are mean intensity, c-fos channel is maximum intensity over all 28 animals. Right: c-fos+ and cort+ cells were counted in the dorsal thalamus of N = 2,6,6,6,8,8 animals for no stim, continuous, bout like, conspecific, cort, and cort & c-fos groups, respectively. Data are mean ± 1SD. c, Normalized bulk c-fos signal for each activity cluster. Bars represent median±1SD, N = 6,8,6,8 animals for ‘No stim’, ‘Continuous’, ‘Bout-like’, ‘Conspecific’, respectively. Individual data points are available in the figure source data. Scale bars: 100 μm.
