Extended Data Fig. 10: Regulation and function of ZBTB46⁺ ILC3s.

a. Representative flow cytometry histogram of RORγt expression in CD4⁺ ILCs from the large intestine of indicated mice. Cells were gated from Lineage⁻CD90.2⁺CD127⁺CD4⁺ cells. b,c. Representative flow cytometry histogram of ZBTB46 expression and quantification of ZBTB46 MFI in CD4⁺ ILCs from the large intestine of indicated mice (n = 4 or 5 mice per group). Cells were gated from Lineage⁻CD90.2⁺CD127⁺CD4⁺ cells. d. Representative flow cytometry histogram of RORγt expression in CCR6⁺ ILC3s from the large intestine of indicated mice. Cells were gated from Lineage⁻CD90.2⁺CD127⁺RORγt⁺CCR6⁺ cells. e,f. Representative flow cytometry histogram of ZBTB46 expression and quantification of ZBTB46 MFI in CCR6⁺ ILC3s from the large intestine of indicated mice (n = 4 or 5 mice per group). Cells were gated from Lineage⁻CD90.2⁺CD127⁺RORγt⁺CCR6⁺ cells. Data are representative of two independent experiments in a-f. Data are shown as the means ± S.E.M. Statistics are calculated by two-tailed unpaired Student’s t-test. g. ZBTB46 defines and regulates ILC3s that protect the intestine.

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