Extended Data Fig. 9: Feature ensembles in AC compared with context ensembles in CA1.
From: Prefrontal feature representations drive memory recall
a, Feature responsive ensembles in AC respond minimally to other features in the same context, shown for reward (left) and aversive (right) feature presentations. b, Quantification of net dF/F activity of feature responsive neurons to all other feature presentations in AC (left) and CA1 (right) from n = 3 mice in reward context. Note: only context responsive neurons are shown (not all recorded neurons). Data are presented either as mean±s.e.m (above) or as heatmap (below), with adjacent column indicating whether the neuron was statistically classified as feature selective (white) or not (black). See Fig. 3f for aversive c, Quantification of net relative response of a feature selective ensemble to other features of the same context (dark) versus the opposite context (light) in AC and CA1 respectively across all mice, each point represents individual mouse (n = 7 mice (t16 = 2.849; *p = 0.023); n = 3 mice CA1 (t16 = 5.035; ***p = 0.0002); Two-way ANOVA with Sidak’s multiple comparison test). d, Performance of SVM to decode reward and aversive trials with all features pooled into either context (n = 3 mice, 9 sessions CA1; 4 mice, 11 sessions AC; Data are mean±s.e.m). e, Schematic of state-space location of different features in an N-dimensional space (N is the number of neurons) and defining separation index as ratio of inter-contextual to intra-contextual distance (right). Separation index for AC (n = 12 sessions) and CA1 (n = 9 sessions) (**p = 0.003; Student’s t-test; data as mean±s.e.m) (left). f, Schematic of FOSTRAP behavioral paradigm. Coronal section of AC shows neurons expressing st-GtACR2 (red) stained with DAPI (blue) with cannula implant (Scale: 500μm, 100μm). Details of statistical analyses in Supplementary Table
