Extended Data Fig. 10: Simultaneous imaging of AC and CA1 during behavior.
From: Prefrontal feature representations drive memory recall
a, Histology of AC and CA1 GRIN lens implant from a representative mouse. Scale = 500μm. b, Anatomical location of each recorded neuron and its activity correlations with motor variables (speed, acceleration) in AC (top) and CA1 (bottom) c, Behavioral performance of dual GRIN implanted and recorded mice, shown as average lick rate on retrieval day 1 (R1) across aversive and neutral features on modified one-day behavioral paradigm (n = 3 mice, *p < 0.023; paired t-test). d, Left: Average fraction of context selective neurons in AC and CA1 during training day T1, with average ensemble size of feature responsive neurons in these regions during retrieval session R1 (right). e, Location of context-selective neurons are evenly distributed throughout field of view in both AC and CA1. f, Top: Proportion of context selective neurons responding (cumulative distribution function) to context onset during training for two mice as a function of latency (Kolmogorov–Smirnov two-tail test, p = 0.015 (m2), p = 0.001 (m3)) (purple-CA1; green-AC), with mean onset time for AC and CA1 (n = 3, paired t-test p < 0.05) Bottom: Same but for retrieval, with feature selective neurons in AC and context selective neurons in CA1 (Kolmogorov–Smirnov two-tail test, p = 0.002(m2), p < 0.0001 (m3)), with mean onset times of AC and CA1 (n = 3 mice, paired t-test, p = 0.01 (Training); p < 0.0001 (Retrieval)). g, proportion of shock responsive neurons active after context-onset (showing first and last trials) as a function of time (cumulative distribution function), and shown separately for each individual mouse. Details of statistical analyses in Supplementary Table
