Extended Data Fig. 1: Behavioral performance during training and retrieval.

a, Latency to the first lick in reward (red), neutral (blue) and aversive (black) context in both, reinforced and probe trials during training. n = 12 mice, 24 sessions. (Two-way ANOVA with Sidak’s post hoc; adjusted *p = 0.029; ***p = 0.001; ****p < 0.0001) b, Lick rate modulation in full cue (AVOT) trials during retrieval (n = 12 mice, 18 sessions; Two-way ANOVA with Sidak’s multiple comparisons; adjusted ***p < 0.005). c, Histology of bilateral inhibition of CA1 throughout training (T1–T3, reinforced trials only, not probe trials) using st-GtACR2 and cannula implant (right, Scale: 1000μm); behavioral performance measured as discrimination index (reward– aversive context lick rate / total lick rate) on probe trials in control (mCherry) vs. opsin (GtACR) cohorts (n = 6 each; Two-way ANOVA with Sidak’s multiple correction, adjusted *p = 0.015, Data are mean±s.e.m) d-e, GRIN lens implant does not affect learning d, lick rate modulation of mice implanted with GRINs in reinforced and probe trials, n = 16 mice, (Two-way ANOVA performed with Sidak’s multiple comparisons, p<adjusted *p < 0.05, ***p < 0.0105, ****p < 0.001) e, Discrimination index in reinforced and probe trials over training days 1–3 (T1–T3); n = 16 mice, Two-way ANOVA with Sidak’s multiple comparison test, adjusted *p = 0.01, Data are mean±s.e.m). Details of statistical analyses in Supplementary Table

Source data.