Extended Data Fig. 7: Randomizing chromosome organization with monastrol decreases mis-segregation bias a.

Quantification of cell survival of RPE1-hTERT cells synchronised, treated for 4 h with monastrol, followed by a washout and shake-off. Experiment was performed three times (scale bar, 5 μm, mean ± s.e.m., two-tailed Fisher’s exact test, n = 120 daughter cells per condition). b. Representative scKaryo-seq results of RPE1-hTERT cells undergone a monastrol washout. c. Quantification of the change in the observed mis-segregation rate compared to expected (4.3%) for Cpd-5 only treated RPE1-hTERT cells versus monastrol plus Cpd-5 treated ones (mean ± s.e.m., two-tailed Fisher’s exact test). d. Quantification of aneuploidy levels of HCT116 either treated with Cpd-5 only or a combination of monastrol and Cpd-5 as described before. Experiment was performed twice for the Cpd-5 treated cells and three times for the monastrol treated ones (mean ± s.e.m., n = 233 and 180 aneuploid cells, respectively). e. As in c, but for HCT116 cells (mean ± s.e.m., two-tailed Fisher’s exact test).