Extended Data Fig. 1: Characterization of RPE1-hTERT mis-segregations.

a. Representative gating strategy for flow cytometry and the DNA content of RPE1-hTERT cells synchronised using palbociclib and RO-3306. Fourth graph on the right shows the DNA content 4 h after washing out RO-3306. b,c, Stills (b) and quantification (c) of the time of alignment in synchronised and MG-132-treated RPE1-hTERT H2B-mNeon cells (scale bar, 5 μm). Experiment was performed in triplicate (mean ± s.d., two-tailed unpaired t-test, n = 60 and 58, respectively). d. Quantification of the time from condensation to full alignment or anaphase onset in DMSO or Cpd-5, respectively. Experiment was performed in triplicate (mean ± s.d., two-tailed unpaired t-test, n = 75). e. Quantification of the segregation error percentages of RPE1-hTERT H2B-mNeon treated with different concentrations of Cpd-5. Three independent experiments were performed (mean ± s.e.m.). f. Quantification of the time from condensation to anaphase onset of cells treated with DMSO or 62.5 nM Cpd-5. Three independent experiments were performed (mean ± s.d., two-tailed unpaired t-test, n = 45 cells).