Extended Data Fig. 2: Characterization of survival after Cpd-5 treatment of multiple cell lines.
From: Nuclear chromosome locations dictate segregation error frequencies
a,b, Representative stills of a RPE1-hTERT H2B-mNeon cell going through mitosis after synchronization and release in 62.5 nM Cpd-5 (a) and quantification of cell death (b). Three independent experiments were performed (scale bar,5 μm, mean ± s.e.m., unpaired t-test). c,d, Stills of BJ-hTERT cells in 31.25 nM Cpd-5 (c) and quantification of cell death (d). Experiment was performed in triplicate (scale bar, 5 μm, mean ± s.e.m., two-tailed Fisher’s exact test, n = 120 daughter cells per condition). e,f, Stills (e) and quantification (f) of human intestinal organoids going through mitosis in the presence of Cpd-5 (scale bar, 5 μm). Two independent experiments were performed (mean ±.s.e.m., two-tailed Fisher’s exact test). g. Graph illustrating sorting strategy of human intestinal organoids to enrich for cells with aneuploidies. Organoids were treated with EdU for 3h, washed and treated overnight with 62.5 nM Cpd-5. EdU-positive G1 cells were sorted.
