Extended Data Fig. 3: Within-host evolution of E. gallinarum in monocolonized mice.
From: Within-host evolution of a gut pathobiont facilitates liver translocation
a–c, Phylogenetic tree of liver and faecal E. gallinarum isolates sampled from mouse 1 (a), mouse 2 (b) or mice 3-5 (c). Reference-based alignments. d, Position, effects, and frequency of mutations at walK, manX, manY, lacE, ypdA, and immR in liver or faecal isolates sampled from each individual mouse. Rows represent mutations and columns represent individual mice. e, Relative abundance of E. gallinarum isolates in the faeces of bi-colonized mice. EGF1-FE4, EGmono6-LV1, or EGmono6-LV10 was co-gavaged with an equal dose of EGmono7-FE2 and samples were collected 4 weeks after co-colonization. See Supplementary Table 7 for a summary of all mutants. n = 3 mice. Representative of two independent experiments. Data represent mean ± SEM. Two-way ANOVA with Sidak’s post hoc test. f, Colony morphologies of EGmono6-LV1, EGmono6-LV10, and EGmono7-FE2 after 48-hour aerobic culture on GAM agar plates.
