Fig. 2: Expression analysis of candidate genes in S. nux-vomica (strychnine producer) and Strychnos sp. (diaboline producer).
Both strychnine and diaboline are derived from the same biosynthetic intermediate, the Wieland–Gumlich aldehyde. a, Expression profiles of identified genes in S. nux-vomica. The expression of each identified gene is represented as the FPKM of S. nux-vomica transcriptomes. Sample sets 1 and 2 represent two biological replicates. b, Co-expression analysis using SnvGO as bait in S. nux-vomica. The circle of dots represents genes co-expressed with SnvGO (Pearson’s r ≥ 0.95; 470 genes in total). c, S. nux-vomica and Strychnos sp. share a common pathway from geissoszhizine 1 to Wieland–Gumlich aldehyde 6. d, Co-expression analysis in Strychnos sp. SpGO, SpNS1, SpNS2, SpNO and SpWS were used as baits. The circle depicts genes co-expressed with all the baits (r > 0.6; 3,999 genes in total). Enlarged and annotated dots in (b and d) represent genes tested in N. benthamiana.
