Extended Data Fig. 1: Functional characterization of SnvGO, SnvNS1 and SnvNS2.

a. Transient expression of SnvGO in N. benthamiana with co-infiltration of geissoschizine 1. Extracted ion chromatograms for akuammicine 3 (m/z [M+H]⁺ = 323.1754 ± 0.05). CrGO was used as a positive control. This experiment was repeated three times with similar results. b. MS/MS (20 to 50 eV) spectra of akuammicine 3 produced in N. benthamiana (blue) compared to standard (red). c. Reaction catalyzed by SnvGO. d. Transient expression of SnvGO, SnvNS1, and SnvNS2 in N. benthamiana with co-infiltration of geissoschizine 1. Extracted ion chromatograms for akuammicine 3 (m/z [M+H]⁺ = 323.1754 ± 0.05, left) and norflurocurarine 4 (m/z [M+H]⁺ = 293.1648 ± 0.05, right). This experiment was repeated three times with similar results. e. MS/MS (20 to 50 eV) spectra of norflurocurarine 4 produced in N. benthamiana (blue) compared to standard (red). f. Reaction catalyzed by SnvNS1 and SnvNS2. EV, empty vector.