Extended Data Fig. 2: Functional characterization of SnvNO.
a. Transient expression of SnvGO, SnvNS1, and SnvNO in N. benthamiana with co-infiltration of geissoschizine 1. Extracted ion chromatograms for norflurocurarine 4 (m/z [M+H]+ = 293.1648 ± 0.05, left) and 18-OH norflurocurarine 5 (m/z [M+H]+ = 309.1567 ± 0.05, right). This experiment was repeated three times with similar results. b. MS/MS (20 to 50 eV) spectra of 18-OH norflurocurarine 5 produced in N. benthamiana (blue) compared to standard (red). c. Reaction catalyzed by SnvNO.
