Extended Data Fig. 9: The structure of HHAT-SHH-N complex.
From: Mechanisms and inhibition of Porcupine-mediated Wnt acylation
a, Representative Superose 6 increase 10/300 GL gel-filtration chromatogram of HHAT-SHH-N complex with Fab3H02. The peak fraction is shown on SDS-PAGE with molecular markers. Each protein is indicated. For gel source data, see Supplementary Fig. 1b. b, Fourier shell correlation (FSC) curve as a function of resolution using RELION-3 output. c, Cryo-EM map of the complex after 3D refinement reveals the mean body of SHH-N protein at the threshold level of 0.003 but not 0.01.
