Extended Data Fig. 2: Protein Purification and data processing of palmitoleoyl-CoA-bound and LGK974-bound PORCN.
From: Mechanisms and inhibition of Porcupine-mediated Wnt acylation
a, Representative Superose 6 increase 10/300 GL gel-filtration chromatogram of PORCN complex with Fab2C11. The peak fraction is shown on SDS-PAGE with molecular markers. Each protein is indicated. For gel source data, see Supplementary Fig. 1a. b, The data processing of palmitoleoyl-CoA-bound PORCN. The cryo-EM 3D classes as well as the mask used for the refinement are shown. The final cryo-EM map after cryoSPARC refinement was sharpened with a B-factor value of −170 Å2. c, Fourier shell correlation (FSC) curve of palmitoleoyl-CoA-bound PORCN map as a function of resolution using cryoSPARC output. d, The data processing of LGK974-bound PORCN. The cryo-EM 3D classes as well as the mask used for the refinement are shown. The final cryo-EM map after cryoSPARC refinement was sharpened with a B-factor value of −130 Å2. e, Fourier shell correlation (FSC) curve of LGK974-bound PORCN map as a function of resolution using cryoSPARC output.
