Extended Data Fig. 2: Activations of MGN:NT, vHPC:NT and PVT:NT terminals evoke both EPSCs and IPSCs in BLA neurons, but IPSCs are not monosynaptic.
From: Neurotensin orchestrates valence assignment in the amygdala
a: Representative images of BLA neurons at lower (left) and higher (right) magnifications used to assay synaptic currents from axon terminals coming from the MGN, the vHPC or the PVT. b: Representative traces of excitatory and inhibitory post-synaptic currents (EPSCs and IPSCs) recorded from putative principal neurons in the BLA upon stimulating ChR2-expressing axon terminals from NT populations in the MGN (left), the vHPC (middle) and the PVT (right). c: The EPSCs had faster onset times than the IPSCs upon stimulation of NT population axon terminals from the MGN (Two-tailed paired t-test, t11 = 3.587, **P = 0.0043), the vHPC (Two-tailed paired t-test, t4 = 3.730, *P = 0.0203) and the PVT (Two-tailed paired t-test, t8 = 4.354, **P = 0.0024). d: Light-evoked EPSCs were monosynaptic as they were still evoked upon stimulation of axon terminals from NT populations of all three regions after the application of TTX+4-AP. Monosynaptic EPSCs were significantly reduced by the application of glutamate blockers for AMPA and NMDA receptors, AP5 and NBQX respectively (One-way ANOVA with Holm-Sidak's multiple comparisons test. The MGN: F(2, 27) = 9.613, TTX+4-AP vs. ACSF: t27 = 1.209, P = 0.2372, effect size = −31.28 ± 28.87; TTX+4-AP vs. AP5+NBQX: t27 = 3.19, **P = 0.0072, effect size = 89.95 ± 28.2; The PVT: F(2,39) = 10.67, TTX+4-AP vs. ACSF: t39 = 2.786, **P = 0.008, effect size = 141.9 ± 50.93; TTX+4-AP vs. AP5+NBQX: t39 = 4.585, ***P < 0.0001, effect size = 233.5 ± 50.93; The vHPC: F(2, 26) = 4.304; TTX+4-AP vs. ACSF: t26 = 2.013, P = 0.0545, effect size = 141.9 ± 70.48; TTX+4-AP vs. AP5+NBQX: t26 = 28.03, *P = 0.0188, effect size = 224 ± 79.91. e: IPSCs were abolished upon application of TTX+4-AP confirming that they are not monosynaptic. There was no detectable positive deflection in the current after application of TTX+4-AP from any cell. n denotes number of neurons in each group. Error bars around the mean indicate s.e.m.
