Extended Data Fig. 3: Immune phenotyping of surviving Adar-/- Mavs-/- Zbp1Zα1α2/Zα1α2 mice and analysis of Adar-/- Zbp1Zα1α2/Zα1α2 embryos.
From: ADAR1 prevents autoinflammation by suppressing spontaneous ZBP1 activation
a, Peripheral blood of 20 week old Adar-/- Mavs-/- Zbp1Zα1α2/Zα1α2 pups and their littermates was analysed for total red blood cell (RBC), white blood cell (WBC) and platelet (PLT) numbers, together with haemoglobin (Hb) and haematocrit (HCT) levels. b, Flow cytometric quantification of numbers of circulating lymphocytes (B cells, CD4 and CD8 T cells, and NK and NKT cells) or myeloid cells (neutrophils, basophils, eosinophils and Ly-6C- and Ly-6C+ monocytes) per µL of blood. c, Gating strategy for flow cytometry analysis in (b). d, Immunoblot analysis of embryonic (E) day 12.5 whole embryo lysates of the indicated genotypes. e, Numbers of embryos resulting from interbreeding of Adar+/- Zbp1+/+ or Zα1α2/Zα1α2 breeding pairs and dissected on the indicated embryonic (E) days. f,g, RT-qPCR analysis of the indicated ISGs and Ifnb (f) or inflammatory genes (g) analysed in E12.5 embryos of the indicated genotypes. Lines in (a,b,f,g) represent the mean; each data point represents an individual mouse; each data point represents an individual mouse; the numbers of mice (n) that were analysed per genotype are indicated in the graph; P values by Mann-Whitney test. For gel source data, see Supplementary Figure 1
