Extended Data Fig. 1: Cryo-EM data processing for SfSTING with c-di-GMP.
From: Cryo-EM structure of an active bacterial TIR–STING filament complex
a, Section of a representative electron micrograph (n = 10,839 from 2 datasets) of SfSTING filaments formed in the presence of c-di-GMP. b, Data processing scheme used to generate c). c, Reconstructions of single and double SfSTING filaments bound to c-di-GMP, coloured by local resolution. d, SfSTING single fibre curvature is evident along the pseudosymmetry C2 axis. L (left) and R (right) monomers are false coloured for clarity. e, SfSTING double fibres make additional (limited) STING to TIR contacts. Lack of sufficiently resolved density for the TIR domains limits analysis of this suprafilament interaction.
