Extended Data Fig. 1
From: Secreted fungal virulence effector triggers allergic inflammation via TLR4
(a) TNF ELISA on supernatants from BMDMs infected for 24 h with the indicated yeasts at MOI = 10. n = six biologically independent samples. Significance determined by one-way ANOVA with Bonferroni test. (b) Intracellular FACS staining for iNOS after 24 h of infection with either C. neoformans or S. cerevisiae at the indicated MOIs. n = three biologically independent samples. (c) RNA-seq heatmap depicting log2 fold changes of the indicated pro-inflammatory genes in BMDMs following 6 h of stimulation. (d) RNA-seq heatmap depicting log2 fold changes of the indicated M2/tolerized genes in BMDMs following 6 h of stimulation. (e) Representative FACS plots of ARG1 and iNOS expression in BMDMs following 24 h of stimulation with PBS, IL-4 (40 ng/ml), or LPS (100 ng/ml) and IFNγ (50 ng/ml). Data are presented as mean values +/– SD. ****p < 0.0001.
