Extended Data Fig. 5: The Mg2+-binding site and conformations of NosD.
From: Molecular interplay of an assembly machinery for nitrous oxide reductase
a, Stereo representation of the C-terminal Mg2+-binding loop in the nucleotide-free state of the NosDFY complex. The cation is octahedrally coordinated in the region from residues D359 to D367, orienting arginine R360 to tightly interact with the membrane-integral NosY subunit. b, Cartoon of NosD bound to the open NosY dimer in the nucleotide-free state of NosDFY. The HMM motif is indicated by a violet disc. c, Cartoon of NosD bound to the closed NosY dimer in the ATP-bound NosF(E154Q) variant.
